Virus identification from CSF and Respiratory Samples

  • Research type

    Research Study

  • Full title

    Retrospective identification of PARV4 and other known and unknown viruses from CSF and respiratory samples from hospitalised UK patients

  • IRAS ID

    154414

  • Contact name

    Philippa Matthews

  • Contact email

    p.matthews@doctors.org.uk

  • Sponsor organisation

    Clinical Trials and Research Governance

  • Research summary

    This is a pilot study, setting out to study the range of viruses present in samples from patients hospitalised in the UK. The study will help to optimise methods for detecting viruses in clinical samples, to determine which viruses are present in our samples of interest, and to inform planning of future studies to investigate the clinical significance of these viruses.

    We are proposing to use left-over samples from Oxford University Hospital's microbiology laboratory. We will identify respiratory and CSF (Cerebro-Spinal Fluid, usually collected by a lumbar puncture test) samples, only after all clinical investigations are complete and the sample would otherwise be discarded.

    We plan to screen these samples using two different methods for detecting viral genetic material: first, to identify viruses belonging to specific viral families, and second to identify any viruses, irrespective of family.

    We are particularly interested to see whether we can detect a virus called PARV4, that was first identified in 2005 using a similar screening approach. There is no clear clinical syndrome associated with this virus, and it is currently not regarded as clinically significant. However, small studies have identified it in CSF and respiratory samples from children in Asia and Africa respectively, prompting us to ask whether we can replicate this in a UK study.

    The technique used for identification of PARV4 shows how valuable it can be to screen for unknown, or unexpected, viruses. This underpins our approach to screening, such that we can potentially identify a broad range of different viruses.

    Collecting a small amount of clinical data (anonymised and accessed from the electronic microbiology record only) will allow us to build up a preliminary picture of which individuals might be at risk of viral infection, and will inform the planning of future studies focusing on the most appropriate patients and samples.

  • REC name

    London - Central Research Ethics Committee

  • REC reference

    14/LO/1077

  • Date of REC Opinion

    28 Jun 2014

  • REC opinion

    Further Information Favourable Opinion