Test-retest operator typical error for tHb-mass measurement.
Research type
Research Study
Full title
The measurement of total haemoglobin mass using the optimised carbon monoxide rebreathing technique: ‘Test–retest’ typical error of the operator.
IRAS ID
174547
Contact name
James Plumb
Contact email
Duration of Study in the UK
1 years, 4 months, 31 days
Research summary
This study is linked to two ongoing NHS REC approved studies in Southampton and University College London regarding the measurement of total haemoglobin mass in patients.
This study is a validation study in healthy volunteers to ensure that our researchers are performing the test accurately.
It is essential for any measurement technique to produce valid and reliable results so that values can be interpreted with confidence. Retest reliability concerns the reproducibility of the observed value when the measurement is repeated. Total haemoglobin mass (tHb-mass) represents the absolute mass of circulating haemoglobin in the body and can be safely, cheaply and reliably measured using the optimised carbon monoxide (CO) rebreathing method. The CO rebreathing method has been shown to produce valid and reliable determination of total haemoglobin mass (tHb-mass) in athletes and healthy individuals. Laboratories measuring tHb-mass should be confident that if the test were to be repeated a few days later, on the same individual, the results would be the same or very similar, all other things being equal. It is important for every laboratory to understand the range of error within their measurement, and this is the aim of this project.
This is a single centre, observational pilot study in healthy volunteers. Participants will have their tHb-mass measured using the optimised carbon monoxide rebreathing technique.
Who?: Healthy adults volunteers (18 years or older).
Where?: University Hospital Southampton- Wellcome trust Clinical Research facility
How?:
Patients perform two tests to measure total haemoglobin mass using the optimised carbon monoxide rebreathing method on two separate days not separated by more than 7 days. This involves one blood sample for haemoglobin concentration per test and the placement of a venous cannula for 3 blood gas samples per test.
Outcome measures: The operator typical error as described by Hopkins et al {Hopkins 2000}
REC name
South Central - Hampshire B Research Ethics Committee
REC reference
15/SC/0496
Date of REC Opinion
6 Aug 2015
REC opinion
Further Information Favourable Opinion