Syntaxin Phosphorylation in human adipose tissue
Research type
Research Study
Full title
Syntaxin phosphorylation in human adipose tissue
IRAS ID
234532
Contact name
James Boyle
Contact email
Sponsor organisation
NHS Greater Glasgow & Clyde
Duration of Study in the UK
2 years, 4 months, 7 days
Research summary
Type 2 diabetes is an increasing problem worldwide. A major aspect of insulin action is to increase glucose uptake by adipose tissue and skeletal muscle. This is achieved through the regulated trafficking of the facilitative glucose transporter GLUT4 from insulin-sensitive intracellular stores, referred to as GLUT4 storage vesicles (GSVs), to the cell surface. Currently our therapeutic options for type 1 diabetes are a 'one size fits all' and do not address the specific underlying molecular abnormalities.
Individuals with insulin-resistance and T2DM exhibit a significant impairment in the ability of insulin to stimulate glucose transport. Numerous studies in cell lines and animal models, as well as influential early studies in humans using now superseded methodologies, have demonstrated various defects in insulin stimulated glucose transport.
Our aim is to evaluate this in human tissue. We currently have access to SGBS cells which are a human cell line from patients with Simpson Golabi Behmel Syndrome, however would like to be able to compare these to human adipose tissue. We would like to further examine the molecular mechanisms of glucose transport, and how these are affected in human tissues, and in particular, human adipose tissue.
We would aim to recruit patients via the Glasgow Weight Management Service who are undergoing weight loss surgery, and via the operating surgeon, would collect a small sample of adipose tissue from the laparoscopic port site. This would then be transported to our laboratory for further analysis.REC name
HSC REC A
REC reference
18/NI/0073
Date of REC Opinion
10 Apr 2018
REC opinion
Favourable Opinion