Study of cow's milk casein in type-1 diabetes
Research type
Research Study
Full title
Validation of an in-house ELISA for circulating antibodies against proteolysis-resistant antigens derived from cow milk caseins in patients with type 1 diabetes
IRAS ID
206715
Contact name
Sandra MacRury
Contact email
Sponsor organisation
University of the Highlands & Islands
Clinicaltrials.gov Identifier
N/A, N/A
Duration of Study in the UK
1 years, 11 months, 31 days
Research summary
Our recent work has suggested that plamsa antibodies against cow's milk casein may trigger autommune responses against beta-cells in type-1 diabetes (T1D) patients who carry the T1D risk genotype called the human leukocyte antigen DQ8 (HLA-DQ8) genotype. Because our initial work recruited 145 T1D patients with mean age of 50 years, we need younger patients with T1D to replicate our initial findings. A total of 350 T1D samples including heparinised plasma and buffy coats will be analysed in this study. These samples were collected through the University of Dundee from T1D patients aged from 16 years to 25 years, and were all unlinked anonymised. Plasma samples will be used to detect IgG antibodies against cow's milk caseins and T1D-associated autoantibodies; buffy coats will be used to extract genomic DNA for genotyping HLA-DQ8 and HLA-DQ2.5. These patients will then be divided into two groups based on whether they carry either DQ8 or DQ2.5; the difference in antibody levels will then be examined between these two groups, and the correlation between the levels of anti-casein and T1D-associated autoantibodies will also be tested.
REC name
East Midlands - Nottingham 2 Research Ethics Committee
REC reference
16/EM/0228
Date of REC Opinion
23 May 2016
REC opinion
Favourable Opinion