Spatial transcriptomics of lung tissue
Spatially resolved host-pathogen interactions across the cystic fibrosis lung
Josephine M Bryant
Genome Research Limited operating as The Wellcome Sanger Institute
Duration of Study in the UK
3 years, 0 months, 0 days
People with cystic fibrosis are highly susceptible to bacterial infections, but many of these infections are extremely resistant to treatment and can persist for decades. We want to understand the host-pathogen interactions that allow the pathogens to survive in the lung and evade treatment. We will apply spatial transcriptomics to retrospectively collected lung tissue from transplant recipients to capture gene expression patterns of both human and pathogen origin.
Samples will be selected from Duke University tissue bank and will be sent to the Wellcome Sanger Institute for DNA and RNA extraction and sequence analysis.
There will be no patient interventions or identifiable information used in this study.
South Central - Berkshire Research Ethics Committee
Date of REC Opinion
8 Feb 2023