Role of denture biofilms in respiratory infection

• Research type

  Research Study

• Full title

  Analysis of denture biofilms and associated salivary parameters in individuals with or at risk of respiratory infection

• IRAS ID

  200133

• Contact name

  David Williams

• Contact email

  WilliamsDD@cardiff.ac.uk

• Sponsor organisation

  Cardiff University

• Duration of Study in the UK

  1 years, 9 months, 30 days

• Research summary

  In the oral cavity, a diverse array of bacteria, yeasts and other microorganisms are present, which can organise to form a community called a biofilm. The most common example of a biofilm in the mouth is dental plaque. The microbial population of the biofilm can alter in health and illness, and may influence disease elsewhere in the body by acting as a reservoir for harmful (pathogenic) microbes. Previous research has linked the development of pneumonias in the vulnerable elderly to the carriage of specific respiratory pathogens within the oral cavity - particularly in denture-wearing individuals. Pneumonia can be difficult to accurately diagnose, often results in hospitalisation, may require aggressive treatment, and can be associated with adverse outcomes including high mortality rates.
  The aim of this study is to compare the composition of oral biofilms in "healthy" denture-wearing individuals in care homes (without current or recent respiratory infection), with patients who have a known respiratory infection. In addition, molecules indicating the local immune response in the oral cavity will be compared between the two groups.
  Samples of saliva and oral biofilm will be collected from patients mouths and dentures for laboratory analysis and characterisation. While a small number of previous studies have observed the presence of respiratory pathogens in the oral cavity, these have focused on identification of specific, known bacteria. This study will use a comprehensive approach, meaning all potential pathogens can be identified, rather than a narrow range. Additionally, indications of infection by analysis of biological markers of inflammation and immune system stimulation may provide insight into the disease process and help develop methods for the earlier diagnosis of respiratory infection.

• REC name

  Wales REC 6

• REC reference

  16/WA/0317

• Date of REC Opinion

  12 Oct 2016

• REC opinion

  Further Information Favourable Opinion