PROTEOMIC ANALYSIS OF CYSTIC FLUID OF CRANIOPHARYNGIOMA IN CHILDREN
Research type
Research Study
Full title
PROTEOMIC ANALYSIS OF CYSTIC FLUID OF CRANIOPHARYNGIOMA IN CHILDREN: A MULTICENTRE STUDY
IRAS ID
114520
Contact name
Benedetta Pettorini
Contact email
Sponsor organisation
Research & Development Alder Hey Childrens Hospital
Research summary
The objective of the study is to characterise the cystic fluid of craniopharyngioma and detect peptides/proteins. These peptides/proteins would be valuable in terms of tumour characterisation, but they might also act as specific markers of disease activity and/or progression. In case of intracystic (within the cyst) treatments, those peptides could also represent markers of response to therapy.
Children (age 0-18 years) with diagnosis of cystic and solid-cystic adamantinomatous craniopharyngiomas will be enrolled in the study. The diagnosis will be made on MRI and it has to be confirmed by histology. Samples of cystic fluid (1-3 mLs) will be collected at time of surgery and stored. In cases of intracystic treatment (Bleomycin, INF-α), two samples of the tumour fluid will be acquired for each patient: the first one before starting the treatment and the second one just after the end of the treatment.
A high performance liquid chromatography electrospray ionization mass spectrometry analysis will be performed. The peptides will be analysed to identify them.
The study will take approximately 18 months to complete. Samples will be collected over a 12-month period. Proteomic batch analysis will be undertaken over a six month period following sample collection to minimise variance in assay and environmental conditions.
REC name
North West - Liverpool Central Research Ethics Committee
REC reference
13/NW/0851
Date of REC Opinion
19 Dec 2013
REC opinion
Favourable Opinion