Protein profiling of fibrinolytics treatment in pleural infection

• Research type

  Research Study

• Full title

  Protein profiling of pleural effusion to unravel the mechanisms of actions of intrapleural t-PA - DNase therapy and increase of fluid drainage in pleural infection.

• IRAS ID

  201710

• Contact name

  Ioannis Psallidas

• Contact email

  Ioannis.Psallidas@ndm.ox.ac.uk

• Sponsor organisation

  University of Oxford

• Duration of Study in the UK

  1 years, 0 months, 0 days

• Research summary

  Pleural infection, is a major global disease and current evidence suggests that it's incidence is increasing in both adult and paediatric populations. It is the UK’s third leading cause of death in women and the fifth leading cause of death in men (15,000 new cases per year). More than 30% of patients with pleural infection either die or require surgery. Drainage of infected fluid is key to successfull treatment. The MIST-2 study showed that intrapleural t-PA - DNase therapy improved fluid drainage and reduced the frequency of surgical referral and hospitalisation duration.

  Our research proposal will for the first time, focus onto the discovery and comprehensive understanding of the molecular pathways that contribute to the improvement of the chest tube drainage. We will analyse an anonymised database of patients obtained by a previous clinical trial (MIST-2 study) from our department. The database holds clinical information of patients and the pleural fluid that will be analysed will be chosen based on them. The sample collection consists of pleural fluids and bloods collected from pleural infection patients, categoriased in different groups according to the intrapleural treatment that they received:
  1.double placebo
  2.t-PA only
  3.DNase only
  4.t-pA and DNase
  

  The ultimate aim of our study is to investigate and elucidate the molecular mechanisms through which t-PA and DNase are inducing the pleural fluid drainage.

  The study will be conducted in University of Oxford research facilities. The study will last for 1 year and there are no interventions for the participants of the study as the investigators will only analyse the stored samples that are held under the license of the Oxford Radcliffe Biobank with participant consent in place for the future use of the samples.

• REC name

  West Midlands - Solihull Research Ethics Committee

• REC reference

  16/WM/0177

• Date of REC Opinion

  14 Apr 2016

• REC opinion

  Favourable Opinion