Protein dosage and carbohydrate co-ingestion on mTORC1 co-localisation

• Research type

  Research Study

• Full title

  The effect of protein and carbohydrate feeding on mTORC1 co-localisation and skeletal muscle signalling in humans, at rest.

• IRAS ID

  156962

• Contact name

  Leigh Breen

• Clinicaltrials.gov Identifier

  14/LO/2255, NREScommiteelondon-camden&islington

• Duration of Study in the UK

  0 years, 2 months, 2 days

• Research summary

  Background: Skeletal muscle is made mostly of protein but these proteins can be made or unmade very quickly, for example bed-rest can result in rapid losses of muscle mass. Retaining muscle mass and function throughout life is important for health (Ruiz, 2009). Therefore, we need to understand how and why proteins are built within healthy muscle, or why they are degraded in situations like disease. One protein is responsible for controlling most of these growth processes and we are looking to understand this proteins action in response to feeding.

  Aim: This study will investigate the effects of consuming a commercially available drink containing 20g of protein, 44g of carbohydrate and 1g of fat on these growth processes, specifically the interaction and position of these proteins within a cell or muscle fiber.

  Hypothesis: The interaction of these growth processes will likely increase in response to this nutrient drink suggesting (although not directly measuring) an increase in muscle protein building, which would eventually lead to muscle growth.

  Design: 8 Young healthy males aged 20-25 will be recruited and after screening and informed consent be provided standardized meals for the 24 hour period before the trial. After an overnight fast, participants will attend the laboratory at 07:00 and weighed in loose clothing prior to cannulation of the forearm vein by a qualified phlebotomist. Blood samples will be obtained every 20 minutes throughout the 4 hour trial. After one hour, the nutrient drink will be consumed, and a small piece of muscle obtained (equivalent to about half a pea) from the quadriceps of one leg under local anesthesia using a Bergstrom needle. Additional biopsies will be obtained at 1 and 3 hour after drink consumption from separate incision sites. After biopsy three, the forearm cannula will be removed and the trial ended.

• REC name

  West Midlands - Black Country Research Ethics Committee

• REC reference

  15/WM/0003

• Date of REC Opinion

  8 Jan 2015

• REC opinion

  Favourable Opinion