PRImary versus Metastasis using Iontorrent Next Generation Sequencing
Research type
Research Study
Full title
PRImary versus Metastasis using Iontorrent Next Generation Sequencing (PRIMINGS)
IRAS ID
157105
Contact name
John Le Quesne
Contact email
Sponsor organisation
University of Leicester
Duration of Study in the UK
1 years, 0 months, 1 days
Research summary
Cancer patients with previous treatment for a primary malignancy frequently undergo biopsy to confirm the presence of metastatic disease. Often the diagnosis of metastatic malignancy is straightforward using conventional techniques of light microscopy and immunohistochemistry. In a subset of cases, however, a definite diagnosis of either metastasis or primary tumour can not be confirmed using conventional methods and this causes uncertainty as to be appropriate course of treatment for the patient.
Targeted next generation sequencing (tNGS) has been shown in case reports to prove a useful tool in this setting. Although these case reports suggest great promise for the clinical application of tNGS, as yet this technology has not been assessed using a representative series of clinical cases.
This project aims to firstly validate this technology on pairs of tumours, both from the same patient and for which diagnosis of the second tumour as either metastasis, or a second primary tumour is unambiguous. Once this method is validated, the test would be applied to pairs of tumours from patients where there is uncertainty as to whether the second tumour represents a primary or metastasis.
REC name
East Midlands - Nottingham 2 Research Ethics Committee
REC reference
14/EM/1265
Date of REC Opinion
1 Dec 2014
REC opinion
Favourable Opinion