Prelim
Research type
Research Study
Full title
Activation markers, inflammatory agents and myocardial ischemia/reperfusion injury
IRAS ID
228295
Contact name
Ever Grech
Contact email
Sponsor organisation
Sheffield Teaching Hospitals NHS Foundation Trust
Duration of Study in the UK
1 years, 2 months, 1 days
Research summary
The aim of the study is to investigate how the level of neutrophils, microvesicles and cytokines (blood cells) are affected by STEMI. This will be compared to levels in healthy volunteers. The main benefit of this study is to gain a better understanding of the causes of coronary heart disease which may lead to improved treatments or prevention in the future.
Samples will be taken from participants undergoing a PCI procedure (pre and post). The samples will be analysed to assess the activity of these blood cells and forms the main proposal of applying for ethical approval within this application. The samples will be transferred once taken to the University of Sheffield following the requirements of the Human Tissue Act by the Investigator.
Healthy volunteers will be recruited in line with the patients in order to identify any correlation under University ethics which is already in place under reference SMBRER310.
Bloods will be analysed at the University of Sheffield by the delegated team member. They will look for neutrophil cell count, microvesicle counts (total as well as multicolour flow cytometry to assess cellular origin), and inflammatory molecule (e.g. IL-8, C5a,) levels using ELISA and/or cytometric bead array. We will compare levels detected in venous samples to those from arterial samples using a paired t-test. In addition, the data from these patients will be compared to data we have/are/will be acquiring from healthy volunteers study under University ethics (SMBRER310). We will not only determine significance of changes in levels detected using, for example, an upaired t-test for plasma levels of IL-8, but also determine if there are any alterations in the cellular origin/ratios of cell-derived/platelet microvesicles in patients compared to healthy volunteers. The type of statistical test used to analyse the data will depend on the parameter(s) to be compared.
REC name
West Midlands - Solihull Research Ethics Committee
REC reference
17/WM/0227
Date of REC Opinion
8 Jun 2017
REC opinion
Favourable Opinion