Pilot of DNA extraction from saliva samples
Research type
Research Study
Full title
Pilot of automated DNA extraction from saliva samples
IRAS ID
239554
Contact name
Rebecca Richmond
Contact email
Sponsor organisation
University of Bristol
Duration of Study in the UK
0 years, 11 months, 27 days
Research summary
Saliva samples have been collected as part of the Head and Neck 5000 cohort (HN5000)(http://www.headandneck5000.org.uk/). This study is investigating factors that influence survival and the psychological impact of living with head and neck cancer. Approximately 5400 participants have donated a saliva sample which will be used to extract DNA.
A potential method to extract the DNA is an automated process using the Tecan Freedom EVO 100 robot. The Bristol Bioresource Laboratories (BBL) have extracted over 23,000 saliva samples using this methodology. The saliva samples for HN5000 were collected in a different way to the saliva samples that BBL have experience in extracting. A pilot study is required to ascertain whether the automated method can successfully extract DNA from the HN5000 saliva samples that is of a good enough quality and quantity for downstream analysis.
As the saliva samples from HN5000 are a precious resource the pilot study will be undertaken using samples from University of Bristol staff. This proposal is therefore requesting ethical approval to obtain saliva samples from staff for this pilot.
REC name
South Central - Oxford C Research Ethics Committee
REC reference
18/SC/0113
Date of REC Opinion
19 Feb 2018
REC opinion
Favourable Opinion