Oxalobacter formigenes – Risk factor for Kidney stone formation in CF

  • Research type

    Research Study

  • Full title

    Oxalobacter formigenes – Risk factor for Kidney stone formation in Cystic Fibrosis.

  • IRAS ID

    186870

  • Contact name

    Rebecca Ilyas

  • Contact email

    rebecca.ilyas@uhsm.nhs.uk

  • Sponsor organisation

    University Hospital South Manchester

  • Duration of Study in the UK

    1 years, 0 months, 1 days

  • Research summary

    We are trying to study bacteria (germs) that may stop people from getting kidney stones. These are called Oxalobacter. These normally live in the bowel and may protect people from getting kidney stones.
    Patients with cystic fibrosis also get kidney stones and we think this may be because they have had antibiotics that remove these bacteria.As Oxalobacter resides normally in the gut and is considered to be healthy gut bacteria.
    We want to find out if cystic fibrosis patients have less Oxalobacter than other people and if this is why they have kidney stones more often. We also want to look at the different types of Oxalobacter in both these groups those with Cystic Fibrosis and those who are the normal population that form kidney stones.
    We will test the levels of oxalobacter in cystic fibrosis patients, by testing their sample of faeces (poo),with special containers provided. The patients will bring these along to their next appointment The samples will then undergo a microbiology culture where levels of o.formigenes will be tested using laboratory techniques such as: polymerase chain reaction inorder to identify whetehr the patient does have O formigenes and if so how much of this bacteria they have.
    The different strains of the bacteria will then be analysed using Enzyme Linked Immunoassay (ELISA).

  • REC name

    East of England - Cambridge Central Research Ethics Committee

  • REC reference

    16/EE/0139

  • Date of REC Opinion

    18 Apr 2016

  • REC opinion

    Further Information Favourable Opinion