Minuet
Research type
Research Study
Full title
Multi-zonal Intraepithelial Neoplasia: Understanding Epithelial Transformation
IRAS ID
284455
Contact name
Julie Bowring
Contact email
Sponsor organisation
Homerton University Hospital NHS Trust
Duration of Study in the UK
0 years, 5 months, 1 days
Research summary
Human Papillomavirus (HPV) is known to affect the lower genital tract in women. Persistent HPV infections are associated with pre-invasive and invasive cancer of the LAGT. Multizonal intraepithelial neoplasia (MZN) is the presence of HPV-related squamous intraepithelial lesions (SIL) in more than one zone of the lower anogenital tract (LAGT). Patients who are severely affected in this way, with high-grade squamous intraepithelial lesions (HSIL), are considered at highest risk of squamous cell cancer (SCC) in the LAGT. DNA methylation and other tests have previously been shown to map to likelihood of progression of HSIL to SCC. Limited data has been published in women with MZN and little is known about the DNA methylation profiles in such cases.
Method
A retrospective analysis of 100+ samples in 20 women with MZN who have developed SCC in at least one zone of the LAGT. DNA methylation and genetic (SNPs) profile, E6/E7 testing will be carried out on the samples. Risk factors including immune suppression, smoking and age will be collected.
Results will be presented and analysed according to changes over time and by 2/3 zone vs 4/5 zone MZN.REC name
London - Queen Square Research Ethics Committee
REC reference
20/PR/0749
Date of REC Opinion
28 Apr 2021
REC opinion
Further Information Favourable Opinion