Kidney Biopsy Single Cell Analysis Version 1.0
Research type
Research Study
Full title
Single cell analysis from single extra cores taken at kidney biopsies
IRAS ID
302077
Contact name
Donald Fraser
Contact email
Sponsor organisation
Cardiff University
Clinicaltrials.gov Identifier
271263, Wales Kidney Research Tissue Bank, Renewal, September 2019
Duration of Study in the UK
2 years, 7 months, 31 days
Research summary
Patients with kidney disease may need to undergo needle biopsy of the kidney for diagnosis. This can be biopsy of the person’s own kidney (native kidney biopsy) or, where a person has undergone kidney transplantation, biopsy of the transplanted kidney (transplant kidney biopsy).\nTypically, one to three passes of the biopsy needle are required to obtain enough tissue, the amount of which is assessed by pathology staff after each pass. In this project, patients will be asked to consent to a single additional pass of the needle after required material for clinical evaluation is obtained.\nThe extra tissue core will be processed and analysed by single cell sequencing. This is a new technique by which one can study the gene expression profile of individual cells in a sample. Technology is developing quickly in this field and it may be possible to perform techniques that give extra information at the same time. The leading example extends on single nuclear RNA sequencing, which is our core technique, to provide additional information on the epigenomic landscape of the cells through a technique called ATAC-seq.\nEthical approval is already in place (via Wales Kidney Research Tissue Bank, WKRTB) for left over biopsy material from consenting patients, together with blood and urine samples, to be used in research. It is proposed that in the current study, when the single cell sequencing is completed, left over material from the biopsy is transferred to WKRTB such that it may be accessed for future studies by the current research team or by other researchers. Such additional work will be subject to application to the tissue bank and use of material according to the usual tissue bank procedures.\n
REC name
South West - Frenchay Research Ethics Committee
REC reference
22/SW/0087
Date of REC Opinion
3 Aug 2022
REC opinion
Further Information Favourable Opinion