Improving DNA preservation of surgical lung specimens
Research type
Research Study
Full title
A laboratory based study to improve DNA and RNA preservation of lung specimens following surgery.
IRAS ID
246195
Contact name
Doris Rassl
Contact email
Sponsor organisation
Royal Papworth Hospital
Duration of Study in the UK
4 years, 11 months, 31 days
Research summary
We want to develop a cohort of specially preserved lung specimens that are optimally prepared for genetic and molecular studies. The samples will be placed into a chemical (PAXgene) that is better for preserving DNA than the chemical we usually use (formalin). Our main reason for wishing to do this is that PAXgene fixed samples are superior for studying DNA and RNA and we wish to develop a bank of tissues more suitable for studies to identify the early genetic changes that may predispose or cause a number of respiratory conditions. Researchers wishing to access these samples will be required to make an application through our existing Tissuebank process.
Based on our previous study we are confident that using PAXgene will not be detrimental or compromise the normal diagnostic tests we perform, but are seeking ethical approval as using PAXgene is different from our regular laboratory practice. The use of PAXgene is key to this process as we need to highly preserve DNA integrity in order to perform the types of genetic sequencing approaches (next generation DNA and RNA sequencing) our collaborators need to use.
REC name
North East - Newcastle & North Tyneside 1 Research Ethics Committee
REC reference
18/NE/0353
Date of REC Opinion
9 Nov 2018
REC opinion
Favourable Opinion