Identification of bacterial 'signatures'

  • Research type

    Research Study

  • Full title

    Identification of bacterial 'signatures' characteristic of intestinal disease

  • IRAS ID

    143550

  • Contact name

    Rachael Rigby

  • Contact email

    rachael.rigby@lancaster.ac.uk

  • Sponsor organisation

    Lancaster University

  • Duration of Study in the UK

    3 years, 0 months, 1 days

  • Research summary

    The exact pathogenic mechanisms underlying inflammatory bowel disease (IBD) currently remain unclear however, there is an accumulating range of evidence which suggest that the intestinal microflora have a direct role in disease onset and pathogenesis. It is estimated that approximately 1000 different species of bacteria live within the colon and usually exist in symbiosis with the host. Alterations in the composition and abundance of the microflora are considered to underpin the development of mucosal inflammation, characteristic of IBD, but research has yet failed to identify a distinct opportunistic pathogen to be responsible for disease onset.
    Novel proteomics based methodologies have recently been utilised to identify various bacterial metabolites, via analysis of urine, indicative of bacterial species within the intestine. Such techniques, termed ‘metabonomics’, will enable profiling of the composition and abundance of intestinal microflora.
    This study aims to employ metabonomics to identify novel bacterial metabolite profiles in the urine which are characteristic of intestinal disease. We will correlate metabonomic data with genomic analysis of bacteria in colonic tissue biopsies, to establish whether the metabonomic profile differs between IBD and healthy controls. Such analyses could provide a novel, non-invasive, and inexpensive diagnostic technique for intestinal diseases.

  • REC name

    North West - Preston Research Ethics Committee

  • REC reference

    14/NW/1429

  • Date of REC Opinion

    12 Dec 2014

  • REC opinion

    Further Information Favourable Opinion