Human salivary O-glycome
Research type
Research Study
Full title
Variation of human salivary O-glycome
IRAS ID
203515
Contact name
Radoslaw P Kozak
Contact email
Sponsor organisation
Ludger Ltd
Duration of Study in the UK
0 years, 11 months, 30 days
Research summary
The study of saliva O-glycosylation is receiving increasing attention due to the potential of glycans for disease biomarkers, but also due to easy access and non-invasive collection of saliva as biological fluid. Saliva is rich in glycoproteins which are secreted from the bloodstream or produced by salivary glands. Mucins which are highly O-glycosylated proteins are particularly abundant in human saliva. Their glycosylation is associated with blood group and secretor status, and represents a reservoir of potential disease biomarkers. This study aims to analyse and compare O-glycans released from whole human mouth saliva collected 3 times a day from a healthy individual over a 5 days period. O-linked glycans will be released by hydrazinolysis, labelled with fluorescence tag (procainamide) and analysed by ultra-high performance liquid chromatography with fluorescence detection (UHPLC-FLR) coupled to electrospray ionization mass spectrometry (ESI-MS/MS).
REC name
London - Surrey Borders Research Ethics Committee
REC reference
16/LO/0883
Date of REC Opinion
18 May 2016
REC opinion
Favourable Opinion