Human Derived Demineralised Bone Matrix and its Correlative Imaging
Research type
Research Study
Full title
Hydrogel in combination with biocides for periodontal therapy and correlative imaging
IRAS ID
195487
Contact name
Araida Hidalgo-Bastida
Contact email
Sponsor organisation
Manchester Metropolitan University
Duration of Study in the UK
5 years, 11 months, 28 days
Research summary
New Tissue engineering and Regenerative Medicine therapies inject stem cells into patients using hydrogels, which are liquid at room temperature but gel at physiological temperature, e.g. after injection into the body, for clinical applications in orthopaedic cases, including osteoporosis.
The problem: researchers and clinicians are limited in hydrogels of non human origin for regenerative medicine treatments. This remains a main concern given the risk of using products of animal origin in patients.
Moreover, scientists do not have reliable techniques to assess efficacy of these treatments, delaying translation from the lab into the clinic. Magnetic resonance imaging (MRI) and computed tomography (CT) can identify changes in organs’ size and shape, but only histological analysis of painful biopsies provide cellular detail. Because of this, new stem cells regenerative treatments are difficult to standardise, as there is not a quality control (QC) tool to verify quality of the graft.
This project will obtain a human DBM hydrogel, from cadaveric bone, at the same time as following up the process using micro-tomography at the Manchester X_Ray facility, and SEM and histology at MMU. Human bone will be scanned in microCT, then incubated in formaldehyde for fixation, scanned again, demineralised, scanned again and then processed for histology as a control to be compared to the human DBM obtained.
The hydrogel,to be used in periodontal therapy, will be screened on its own with some pathogenic bacteria to identify if it is antimicrobial. Later, the hydrogel will be charged with some natural and chemical compounds and its antimicrobial properties will be assessed. Last, Mesenchymal Stem Cells and dental pulp will be seeded into scaffold to verify if the antimicrobial properties are modified by the presence of the cells. In parallel, the suitability of the hydrogel for the regeneration of periodontal tissues will be explored with cultures in osteogenic media.
REC name
East of England - Essex Research Ethics Committee
REC reference
16/EE/0109
Date of REC Opinion
19 Apr 2016
REC opinion
Further Information Favourable Opinion