How does infection cause peritoneal dialysis treatment failure?
Research type
Research Study
Full title
Investigating the mechanism of bacteria induced peritoneal dialysis treatment failure
IRAS ID
261952
Contact name
Sara Namvar
Contact email
Sponsor organisation
University of Salford
Clinicaltrials.gov Identifier
1, Investigating the role of mesothelial cells in peritoneal fibrosis
Duration of Study in the UK
1 years, 0 months, 0 days
Research summary
This study will investigate the gene expression changes occurring in mesothelial cells following peritoneal dialysis and related infection. It is hypothesised that mesothelial gene expression changes that come about following peritoneal dialysis and infection contribute to peritoneal fibrosis and ultimately treatment failure. If we can understand the molecular changes contributing to this fibrosis we can develop novel biomarkers and treatment options. We will collect peritoneal effluent cells that would ordinarily be disposed from patients first starting peritoneal dialysis and then again 3 months and/or 6 months later. Collection of follow-up samples in the same patients will minimise the number of samples required overall by eliminating the degree of variability. We anticipate that as many as 10% of the patients we recruit onto our study will have an episode of peritonitis.
We intend to collect cells at the time-points stated, and then either collect RNA/protein directly or grow them in the laboratory so that we can expose them to dialysis/infection and then monitor changes experimentally. The key areas that we will investigate include assessing the early gene and protein expression changes that occur in response to dialysis alone or those more dramatic changes that we anticipate will come about because of infection.REC name
HSC REC B
REC reference
19/NI/0131
Date of REC Opinion
23 Jul 2019
REC opinion
Further Information Favourable Opinion