Host Transcriptomic Changes During Clinical Infection With Ebola Virus
Research type
Research Study
Full title
Host Transcriptomic Changes During Clinical Infection With Ebola Virus
IRAS ID
189909
Contact name
Andrew Bosworth
Contact email
Sponsor organisation
University of Sheffield
Duration of Study in the UK
2 years, 1 months, 11 days
Research summary
This study aims to study the changes in host cells associated with infection with Ebola Virus. Samples used in this study will be those previously submitted for diagnosis and viral load monitoring during the course of an infection with Ebola Virus in a UK patient. Part of the subsequent analysis of this case included the next-generation sequencing of patient samples to confirm Ebola Virus infection and profile changes in the virus. Simultaneously raw data was collected which can be used to determine host gene expression changes which occurred during infection.
In the first instance this data will be utilized to identify changes and attempt to associate these with how advanced the infection had progressed, the viral load at the time of sample taking, and the Ebola Virus genome changes identified in the post-diagnosis analysis.
This will mean preliminary data can be collected without needing to make use of patient-derived material stored after diagnosis was performed.
Additional samples are currently planned as part of patient follow up as decided by the clinical care team and laboratory advisors. These samples will include blood which would act as a background control, as the patient has long-since recovered from infection. Another of these samples will be joint aspirate, which could help identify the cause of Post-Ebola Virus Disease syndrome observed in around 50% of survivors.
No sample will be specifically collected for this study, and will only be collected as part of diagnosis and follow up as determined by the clinical care team and laboratory medical advisors. All use of data collected from previously collected patient samples, and from new samples submitted for diagnosis, will be with the written consent of the patient.
REC name
North West - Greater Manchester West Research Ethics Committee
REC reference
15/NW/0744
Date of REC Opinion
11 Sep 2015
REC opinion
Favourable Opinion