Generation of multi-lineage human-relevant glioblastoma model
Research type
Research Study
Full title
Generation of multi-lineage human-relevant glioblastoma model: Defining a regulatory phenotype for caveolin-1 within and between neoplastic and myeloid cells of the GBM microenvironment.
IRAS ID
300507
Contact name
Mark Gumbleton
Contact email
Sponsor organisation
Cardiff University
Duration of Study in the UK
1 years, 1 months, 26 days
Research summary
Glioblastoma (GBM) is an aggressive brain tumour, invasive and displaying considerable resistance to drug-/radiation-therapies. Tumour recurrence after surgery is common with patient survival ca 5% at 5 years post-diagnosis.
A tumour’s microenvironment (TME) has profound effects upon tumour aggressiveness and treatment response. GBM comprise a substantial proportion of non-cancerous/brain-resident cells as well infiltrating immune cells from the blood. Cancer cell-TME interactions are complex and require human-relevant research models. Tissue organoids, effectively tumour slices, are increasingly used but are deficient in studies of ‘tumour’ progression, where a defined TME is sought or where ‘tumour’ evolution and responsiveness to therapy is to be examined using genetic interventions.
Using stem-cell technology, this proposal aims to establish self-organising “mini-brains” (organoids) which bridges a gap in existing in-vitro 3D culture systems of brain tumours. It will incorporate patient cancer cells together with the patient’s own TME and facilitate wider study of immune therapies outside the in-vivo animal scenario.REC name
Wales REC 4
REC reference
22/WA/0026
Date of REC Opinion
8 Mar 2022
REC opinion
Further Information Favourable Opinion