Generation of Dental Pulp-like tissue
Research type
Research Study
Full title
Regenerative Endodontic Techniques for The Management of Non Vital Immature Permanent Teeth: Generation of Dental Pulp-like tissue Using Decellularized Natural Pulp tissue scaffold and Dental pulp stem cells – Feasibility Study
IRAS ID
212756
Contact name
HAYAT ALGHUTAIMEL
Contact email
Sponsor organisation
University of Leeds
Duration of Study in the UK
4 years, 0 months, 31 days
Research summary
The purpose of this research is to investigate the feasibility and viability of a novel decellularized natural pulp tissue scaffold by attempting to regenerate pulp-like tissue using the scaffold itself, stem cells and growth factors.
Method:
•Study no.1:
1)Human dental pulp will be harvested from teeth donated for research purpose immediately after extraction under local or general anesthetic in Leeds dental institute with full written consent from the patient and/or legal guardian. the age and sex of the patient will be recorded along with the tooth notation for record-keeping purpose but all teeth will be anonymised prior to distribution to the researcher.
2)The pulp tissue after that will be decellularized following the protocol which has been described by Wilshaw et al, 2008. The process of Decellularization means sequential application of chemicals to remove all cells and genetic contents in order to produce tissue that mimic the pulp extracellular matrix.
3)After that, a number of tooth slices will be prepared by making a 3 mm thick, horizontal cut in the cervical region of the tooth using accutom cutting water-cooling machine to give a ring shape samples with a canal in centre.
4)Following preparation of the tooth slices, the decellularized scaffold will be placed in the canal of the tooth slices and then seeded with stem cells and growth factor.
5)The tooth slices/scaffold model will be then placed in a culture media for several days to investigate the viability, cellular attachment and the cellular proliferation.•Study no.2:
1)The first four steps of study no.1 will be repeated. After that, the tooth slices/scaffold model will be implanted in to the the subcutaneous space of the dorsum of immunodeficient rats.
2)After 4 weeks, the tooth slices/scaffold model will be retrieved and then processed for histology, immunohistochemistry and electron microscopy examination.REC name
East Midlands - Nottingham 2 Research Ethics Committee
REC reference
17/EM/0040
Date of REC Opinion
10 Mar 2017
REC opinion
Further Information Favourable Opinion