Generating human colorectal organoids as an enabling technology
Research type
Research Study
Full title
Generating human colorectal organoids as an enabling technology for investigating phenotypic effects of small molecules, nutriceuticals and gene editing approaches
IRAS ID
277768
Contact name
Malcolm G Dunlop
Contact email
Sponsor organisation
University of Edinburgh (ACCORD)
Duration of Study in the UK
5 years, 0 months, 1 days
Research summary
Colorectal cancer (CRC) is the 2nd most common cause of cancer death, accounting for >16,000 deaths in the UK. Understanding how CRC develops is key to prevention and early detection. The aim of this study is the generation of models in the laboratory directly from normal human bowel tissue and from tumours (polyps and cancers). These models, called organoids, will enable us to assess the effect of small molecules, including drugs (e.g. aspirin, metformin, vitamin D). It will also enable insights from genetic studies (genome-wide association studies, sequencing and other studies) that identify key genes causing CRC to be used to mimic those observed effects in human tissue. We will do this using gene-editing approaches to alter the genetic code of the organoids (primarily using a technique called CRISPR-Cas9 editing). By altering the genetic code at specific locations and then observing differences in morphology, behaviour and response to treatment, this will test the influence of genetic factors on risk of development of CRC. We will also biobank these organoids for future use as new genes, agents and other discoveries become available.
REC name
South East Scotland REC 01
REC reference
20/SS/0038
Date of REC Opinion
29 May 2020
REC opinion
Further Information Favourable Opinion