Effects of regional ischaemia and light on NO and Sulphide signalling
Research type
Research Study
Full title
Effects of Regional Ischemia and Light on Circulating biomarkers of NO and Sulphide Signalling
IRAS ID
151677
Contact name
John Pappachan
Contact email
Sponsor organisation
Univeristy Hospital Southampton NHS Foundation Trust
Research summary
Ischemic pre-conditioning is a poorly understood process with a proven clinical benefit. Remote ischaemia leads to protection against low oxygen levels, though mechanisms which are poorly understood even though the practise was first discovered 30 years ago.
This project will be looking into NO and Dihydrogen Sulphide signalling pathways as potential mechanisms for the protective changes, as these are both important signalling molecules and their activity is linked to oxygen availability. Microcirculatory changes will also be used as direct measurements of clinical status.
Another factor that will be investigated is light and its effect on ischemic pre-conditioning. Blue light, used clinically in paediatrics and being used here due to the already well documented link between NO and light, will be utilised in a cohort of patients to determine whether it has an effect on metabolites.
Both clinical measure, such as microcirculatory flow, and biochemical markers, NO and H2S, will be measured in this longitudinal study using standard protocols. Blood samples will be taken before and at set points after the remote ischemic pre-conditioning and changes in metabolites will be determined.REC name
South Central - Berkshire B Research Ethics Committee
REC reference
14/SC/1214
Date of REC Opinion
16 Oct 2014
REC opinion
Further Information Favourable Opinion