Development of immortalised primary erythroid cell lineages
Research type
Research Study
Full title
The development of immortalised primary erythroid cell lineages with different Sickle Cell genotypes.
IRAS ID
218416
Contact name
David Rees
Contact email
Sponsor organisation
King's College London School of Medicine
Duration of Study in the UK
2 years, 0 months, 0 days
Research summary
Sickle Cell Disease is a condition that is caused by genetic changes to the beta globin gene in erythroid (red) cells of the blood. This variation causes the red cells to change shape and polymerise and this process leads to blockage of small blood vessels causing pain and damage to the affected tissue or organ. This sickling process only typically happens in mature red cells expressing adult haemoglobin. To date, the study of this sickling process is somewhat limited by the lack of an immortalised erythroid cell lineage which is maintained in a precursor state, but which can then be differentiated at will to produce mature (enucleated) red blood cells. \nWe propose to use blood samples from a small number patients with various types of sickle cell disease and, using recently developed biological techniques, manipulate the blood cells to become an immortalised erythroid cell lineage. Once established, this will allow further laboratory studies that we hope will yield great insights into the Sickle Cell pathological process.
REC name
North of Scotland Research Ethics Committee 1
REC reference
18/NS/0005
Date of REC Opinion
15 Feb 2018
REC opinion
Further Information Favourable Opinion