Characterisation of the developing brain from surgical specimens
Research type
Research Study
Full title
Characterisation of the developing brain from surgical specimens.
IRAS ID
249231
Contact name
Thomas Santarius
Contact email
Sponsor organisation
Cambridge University Hospitals NHS Foundation Trust and the University of Cambridge
Duration of Study in the UK
0 years, 11 months, 30 days
Research summary
The remarkable cellular diversity of the human brain enables underlies our complex behaviour and vulnerability in many neurological illnesses. Almost half of the cells in the human brain are glia, but we know little about their molecular and functional heterogeneity. Glial cells are now recognized as potent regulators of synapse formation and refinement during brain development and increasingly implicated in neurological diseases. The cerebral cortex provides an attractive and disease relevant brain region to study glial-neuron interactions.
However, existing studies provide a limited understanding of the spatiotemporal molecular profiles of different human glial cell types in the paediatric brain.Study aim: We aim to map the human glial and neuronal transcriptomes in the cerebral
cortex throughout paediatric development using a combination of single cell/nuclei RNA-
sequencing (RNAseq) and large-scale single molecule fluorescent in situ hybridization
(smFISH).REC name
Yorkshire & The Humber - Bradford Leeds Research Ethics Committee
REC reference
19/YH/0274
Date of REC Opinion
12 Feb 2020
REC opinion
Further Information Favourable Opinion