Analysis of perturbed (test) and non-perturbed (control) human samples
Research type
Research Study
Full title
Physiological, biochemical and molecular analyses of primary somatic human stem and progenitor cells in response to chemicals, pharmacological agents or genetic perturbation for research and development purposes in Cellular Operations, a division of the Wellcome Sanger Institute.
IRAS ID
283472
Contact name
Andrew Bassett
Contact email
Sponsor organisation
The Wellcome Sanger Institute
Duration of Study in the UK
6 years, 4 months, 9 days
Research summary
Summary of Research
The Division of Cellular Operations is a group of core scientists which serve to facilitate and optimise the Sanger Institute’s Faculty programmes of research. A large part of the Cellular Operations remit is developing and optimising tissue/cell culture assays including gene targeting and large scale screening. Improving efficiency of standard assays and development of novel scientific approaches, as a group, we are a core hub of expertise and extremely active in delivering Faculty science. Modern tissue culture techniques have been able to establish and improve the long term culture of primary human cells and tissues including the in vivo expansion of rare somatic stem and progenitor cell populations. These methods are under continuous development by the global scientific community. Importantly, any biological or scientific discoveries determined through the use of primary tissues, provide a more clinically tractable source of data than standard cell lines and represent an accompaniment to data from model organisms.We aim to study the effects of chemical, pharmacological or genetic perturbation and variation on the physiological, biochemical and molecular characteristics of somatic human primary stem (non-embryonic), progenitor (developing) and terminally differentiated (fully developed) cells from commercial suppliers or NHS Blood and Transplant (NHSBT). In particular we aim to drive the development of existing protocols with an aim to improving assay efficiencies, cost efficiencies and maximise the scientific return on these valuable resources. For all of the proposed studies genetically identical (isogenic), non-perturbed (control) samples will be assayed alongside perturbed (test) samples for direct comparison and protocol development. Briefly the studies will be comprised of two parts;
i) optimisation and standardisation of techniques and conditions in non-perturbed samples.
ii) comparison of perturbed (test) and non-perturbed matched (control) samples.
Summary of Results
n/a (study not started)REC name
North of Scotland Research Ethics Committee 2
REC reference
20/NS/0079
Date of REC Opinion
25 Jun 2020
REC opinion
Favourable Opinion