Abundances of enzyme and transporters in kidney tissue v1
Research type
Research Study
Full title
Quantification of kidney enzyme and transporters using proteomics and estimation of kidney microsomal protein content and proximal tubule cell number v1
IRAS ID
137360
Contact name
Daniel Scotcher
Contact email
Sponsor organisation
University of Manchester
Research summary
Pharmacokinetic research is the study of what the body does to a drug, and is an essential process that continues throughout the discovery, development and post-marketing surveillance of drugs to improve patient safety and treatment outcome. Major contributions of pharmacokinetic research come from laboratory based experiments, as well as computational modeling and simulation. This can be applied to investigate pharmacokinetics of drugs in certain situations, such as in different population groups or during administration of multiple drugs. Accurate information about the physiology and anatomy of the human body is of great benefit to link the results from experiments with computational models used to simulate drug pharmacokinetics. This also includes differences between individuals in the same and different population groups (i.e. biological variability), for example differences due to age, gender, ethnicity, and disease.
We will take measurements of physiological aspects of the human kidney. The measurements will be made in ‘normal’ kidney tissue taken from multiple kidneys removed during nephrectomy surgery. This will allow for baseline values to be established for the healthy population, and will enable us to investigate the variability between individuals and certain sub-groups (e.g. is there any difference between male and females?). Establishing baseline values in normal tissue can be used for future projects which may investigate other population groups (e.g. diseased or non-functional kidney). This would have potential application in stratified and/ or personalised medicine approaches.
We will use state of the art methods to measure various physiological aspects of the human kidney, which include the abundance of certain enzyme and transporters (which are known to interact with drugs), as well as the number of proximal tubule cells, in human kidney. Subsequent experiments will use isolated cells and sub-cellular preparations from human kidney to investigate the interaction of certain drugs with the enzymes and transporters.REC name
London - Camberwell St Giles Research Ethics Committee
REC reference
13/LO/1896
Date of REC Opinion
26 Nov 2013
REC opinion
Favourable Opinion