A combination of doublestaining to aid in grading follicular lymphoma
Research type
Research Study
Full title
The use of a combination of double staining immunohistochemistry to determine if specific staining patterns of follicular dendritic cell meshworks aid in grading follicular lymphoma
IRAS ID
225461
Contact name
Ayoma Attygalle
Contact email
Sponsor organisation
The Royal Marsden Foundation NHS Trust
Duration of Study in the UK
1 years, 0 months, 0 days
Research summary
The main aim of this study is to determine if there are specific staining patterns of Follicular Dendritic Cells (FDC) using CD21 and CD23 immunostains in the different grades of FL, and whether this would be of use to supplement histological grading currently in use. A secondary aim would be to determine if the staining pattern of FDC in partially colonised follicles differs from normal follicles and those that are entirely neoplastic when present in a single lymph node section.
This study will take place at The Royal Marsden Hospital Histopathology Laboratory, and will involve the use of tissue blocks of patients with known Follicular Lymphoma (FL). This information will have to be retrieved from the Royal Marsden Patient System, to ensure the correct number of cases is retrieved, with the appropriate diagnosis.
A total of 75 cases where a diagnosis of FL has previously been made will be retrieved from the histopathology archives in the department of which 30 of will be grade I/II, and 30 will be grade IIIa, and 15 grade IIIb. In addition, 10 cases where a diagnosis of reactive follicular hyperplasia has previously been made will also be included for comparison.
There will be no direct patient involvement; therefore patients will not be undergoing any specific processes. All data gained from this study will be anonymised and there will be no exposure of patient details.
This will aim to identify if double staining is able to aid the grading of FL, therefore can be used more frequently, to allow pathologists to carry out these stains at ease, to enable grading of the diagnosis to be made more promptly. Therefore allowing doctors to direct the patient’s treatment in accordance with the diagnosis made more swiftly.REC name
London - Queen Square Research Ethics Committee
REC reference
18/LO/0147
Date of REC Opinion
16 Jan 2018
REC opinion
Favourable Opinion